Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential
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منابع مشابه
Isolation of Plasma Membrane Fragments from Hela Cells
A method for isolating plasma membrane fragments from HeLa cells is described. The procedure starts with the preparation of cell membrane "ghosts," obtained by gentle rupture of hypotonically swollen cells, evacuation of most of the cell contents by repeated washing, and isolation of the ghosts on a discontinuous sucrose density gradient. The ghosts are then treated by minimal sonication (5 sec...
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HeLa cell plasma membranes have been purified after binding cells to polylysine-coated polyacrylamide beads. Cell attachment to beads and membrane recovery were maximal in a sucrose-acetate buffer, pH 5.0, at 25 degrees C. Measurements of ouabain-sensitive NaK-adenosine triphosphatase, membrane-bound 125I-wheat germ agglutinin, and chemical analyses showed that membranes on beads were of compar...
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0022-0728/$ see front matter 2009 Elsevier B.V. A doi:10.1016/j.jelechem.2009.01.002 * Corresponding author. Tel.: +1 512 471 3761; fax E-mail address: [email protected] (A.J. Bard) The viability and activity of HeLa cells were probed using scanning electrochemical microscopy (SECM). The feedback generated by HeLa cells during scanning depends on the electrochemical mediator. Living HeLa c...
متن کاملEvaluation of the effects of paederus beetle extract and gamma irradiation on HeLa cells
Objective(s):Cervical cancer is a malignancy that is the second most common cause of death from cancer in women throughout the world. Paederus beetle (Paederus fuscipes) extract (PBE), contains bioactive compounds such as pederine which has cytotoxic properties and blocks DNA and protein synthesis at very low concentrations. In this investigation we tried to determine the effects co-treatment w...
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ژورنال
عنوان ژورنال: Cogent Engineering
سال: 2019
ISSN: 2331-1916
DOI: 10.1080/23311916.2019.1614756